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The β-glucosidase gene for esculin hydrolysis of Yersinia enterocolitica is a suitable target for the detection of biotype 1A strains by polymerase chain reaction.

Please note: As of 1 July 2025, the New Zealand Institute for Public Health and Forensic Science (PHF Science) is the new name for the Institute of Environmental Science and Research (ESR). Research and reports published prior to this date may reference the organisation’s former name.

Abstract

Detection of Yersinia enterocolitica (YE) in food is still challenging, as the species is readily overgrown by other bacteria. The aim of the study was to identify a reliable Polymerase Chain Reaction (PCR) detection target for YE biotype 1A (BT 1A), which may cause human infections. Esculin hydrolysis (attributed to the production of ß-glucosidase) is a recognized differential biochemical reaction for the identification of YE BT 1A compared with other YE biotypes. A multiplex PCR should be developed allowing the detection of all YE biotypes in food.

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