Demonstration of the presence of plasmid-mediated AmpC β-lactamase genes by multiplex PCR.
The test is appropriate for cefoxitin-resistant E. coli, Klebsiella spp and Salmonella spp. Prior to performing the PCR, ESR will screen the isolate for AmpC β-lactamase using a boronic acid double-disc synergy test.
|
Area |
Health Science |
|
Lab name |
Antibiotic Reference Laboratory |
|
Lab location |
Kenepuru Science Centre, Porirua |
|
Test name |
Plasmid-mediated AmpC β-lactamase gene PCR |
|
Organisms |
Escherichia coli, Klebsiella spp and Salmonella spp |
|
Requested for surveillance |
no |
|
Notice required |
no |
|
Routine turnaround time |
10 working days for reference specimens |
|
Sample requirements |
Pure culture |
|
Media requirements |
A slope of any medium that supports the growth of the organism |
|
Pre-transport requirements |
Ambient temperature |
|
Transport requirements |
Ambient temperature |
|
Unacceptable sample types |
Clinical specimens will not be accepted. |
|
How results reported |
The results will state if a plasmid-mediated AmpC β-lactamase was detected, and if so, what group was detected |
|
Test form number |
PHFS0647 |
|
Referral link |