|
Area |
Health Science |
|
Lab name |
Enteric Reference Laboratory |
|
Lab location |
NCBID Wallaceville |
|
Test name |
Escherichia coli Shiga-toxin gene detection and serotyping |
|
Organisms |
E. coli |
|
Turnaround |
10 days |
|
Requested for surveillance |
yes |
|
Notice required |
no |
|
Sample requirements |
Pure culture of an stx positive isolate or a mixed culture from an stx positive specimen. |
|
Media requirements |
Nutrient agar slope for cultures. BVCC and STEC-CHROM agar for mixed cultures. MacConkey or GN broth additionally recommended for patients at risk of HUS. |
|
Pre-transport requirements |
Solid Media should be kept chilled until shipping. Broths should be shipped as soon as possible after growth and should be kept chilled. |
|
Transport requirements |
Ambient temperature |
|
How results reported |
If positive: E. coli serotype and toxin/virulence genes profile. If negative, the isolate will be reported as negative for verocytotoxin. |
|
Test form number |
PHFS0647 |
|
Referral link |